yoaJ [2018-09-21 10:26:49]

bacterial expansin, binds cellulose, required for the colonization of maize roots

locus: BSU18630

pI: 9.45

mw: 25.49 kDa

protein length: 232 aa

gene length: 696 bp

function: interaction with plant roots

product: expansin

essential: no

synonyms: EXLX1

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.4|Lifestyles/ miscellaneous]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 2,032,927 → 2,033,625

Phenotypes of a mutant

• strongly reduced ability to colonize maize roots [Pubmed|18971341]

The protein

Protein family

• expansin [Pubmed|18971341]

Structure

• [PDB|3D30] [Pubmed|18971341]

[SW|Localization]

• extracellular [Pubmed|18971341]

Expression and Regulation

Operons

• genes: [gene|17094CBA2DDFB82B5FC064AADB85DAB60A15B2C0|yoaJ]
  description: [Pubmed|12354229]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • induced upon iron starvation ([protein|search|Fur]) [Pubmed|12354229]

Biological materials

Mutant

• MGNA-A839 (yoaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/839 NBRP B. subtilis, Japan]
• BKE18630 (Δ[gene|17094CBA2DDFB82B5FC064AADB85DAB60A15B2C0|yoaJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTGGTCCTCCTCAAA, downstream forward: _UP4_TAAAAAATACGAAACAGCGG
• BKK18630 (Δ[gene|17094CBA2DDFB82B5FC064AADB85DAB60A15B2C0|yoaJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTGGTCCTCCTCAAA, downstream forward: _UP4_TAAAAAATACGAAACAGCGG

References

Reviews

• 25833181,27365165

Original publications

• 19058186,15604683,16984999,22927418,18971341,21454649,12354229,23053073,24755657,22949138,26521249,26751268,27365165